Effect of non-thermal millimeter electromagnetic radiation on thermodynamic parameters of the binding of ligands with nucleic acids

S. N. HAKOBYAN; V. P. KALANTARYAN; Y. S. BABAYAN

S. N. HAKOBYAN Department of mechanical engineering, Armenian State University of Engineering
V. P. KALANTARYAN Department of Microwave radiophysics and telecommunications, Yerevan State University
Y. S. BABAYAN Department of Physics and Electrotechnics, Yerevan State University of Architecture and Construction

Keywords: DNA – synthetic polyribonucleotide – mitoxantrone – doxorubicin – netropsin – Hoechst 33258

Abstract

It has been shown that intercalating (mitoxantone, doxorubicin) and non-intercalating (netropsin, Hoechst 33258) ligands form more strong complex with nucleic acids irradiated by resonant frequencies (64,5 and 50.3 GHz) for oscillations of water structures. Under the effect of the mentioned non-thermal millimeter waves a dehydration of nucleotides occurs, which results in increasing of the binding constant of ligands with irradiated double-stranded nucleic acids (NA). Calculations showed that as a result of irradiation, the enthalpy (H) and entropy (S) of the binding of ligands to NA has been changed. However the value of H is mainly being changed for intercalators, and the value of S - for non-intercalators.

Author Biographies

S. N. HAKOBYAN, Department of mechanical engineering, Armenian State University of Engineering

Ս. Ն. Հակոբյան

V. P. KALANTARYAN, Department of Microwave radiophysics and telecommunications, Yerevan State University

Վ. Պ. Քալանթարյան

Y. S. BABAYAN, Department of Physics and Electrotechnics, Yerevan State University of Architecture and Construction

Յ. Ս. Բաբայան

babayanyura@gmail.com