Interaction of different intercalators with DNA in three-component systems

A. P. ANTONYAN; M. A. TOROSYAN; A. T. KARAPETYAN

A. P. ANTONYAN Department of Biophysics, Faculty of Biology, Yerevan State University
M. A. TOROSYAN Department of Physics and Electrical engineering, Yerevan State University of Architecture and Construction
A. T. KARAPETYAN Department of Physics and Electrical engineering, Yerevan State University of Architecture and Construction

Keywords: DNA – ethidium bromide – mitoxantrone – joint binding – melting parameters

Abstract

The study of the joint binding of the intercalating compounds ethidium bromide (EtBr) and mitoxantrone (MTX) with DNA has been carried out. Melting parameters of the three-component system EtBr-DNA-MTX were obtained. Changes in the melting parameters of the complexes revealed that EtBr binds by several modes with ds-DNA both in the presence and in the absence of another intercalator. In the absence of EtBr, MTX binds to ds-DNA by semi-intercalation mode. At the joint binding of EtBr and MTX with ds-DNA a competition between ligands emerges, though the effect of EtBr on the melting parameters of the complexes becomes dominant.

Author Biographies

A. P. ANTONYAN, Department of Biophysics, Faculty of Biology, Yerevan State University

Ա.Պ. Անտոնյան

M. A. TOROSYAN, Department of Physics and Electrical engineering, Yerevan State University of Architecture and Construction

Մ.Ա. Թորոսյան

A. T. KARAPETYAN, Department of Physics and Electrical engineering, Yerevan State University of Architecture and Construction

Ա.Թ. Կարապետյան