Interaction of Meso-tetra-(4N-allylpyridyl) porphyrin and its Cu-, Co- and Zn- containing derivatives with DNA

V. G. BARKHUDARYAN; G. V. ANANYAN

V. G. BARKHUDARYAN Department of Molecular Physics
G. V. ANANYAN Yerevan State University

Keywords: Porphyrin – ct-DNA – Viscometry – intercalation – outside binding – partial intercalation

Abstract

The influence of water soluble cationic meso-tetra-(4N-allylpyridyl) porphyrin (H2TAlPyP4) and its metal complexes with Cu-, Co- and Zn- on hydrodynamic and spectral behaviour of ct-DNA solutions has been studied by viscometry and UV-VIS spectrophotometry methods. The results obtained were compared with the results of previously conducted similar studies on meso-tetra-(3N-allylpyridyl) porphyrin (H2TAlPyP3). It has been shown that the change in position of peripheral radicals on pyridylic ring has absolutely no effect on rules of interaction of investigated porphyrins with DNA in case of outside binders CoTAlPyP4 and ZnTAlPyP4. Planar porphyrin H2TAlPyP4 interacts with DNA predominantly by intercalation mode at low relative concentrations of r (r = [Porphyrin] / [DNA]) and by external binding mode at high values of r. Unusual behaviour shows CuTAlPyP4, which interacts with DNA via non-classical (partial) binding mode. It was shown that H2TAlPyP3 and its metal complexes bind to DNA much more intensely than H2TAlPyP4 and its metal complexes.

Author Biographies

V. G. BARKHUDARYAN, Department of Molecular Physics

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Vbarkhudaryan@gmail.com

G. V. ANANYAN, Yerevan State University

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