Denaturing gradient gel electrophoresis (DGGE) profiles of the partial 16S rRNA genes defined bacterial population inhabiting in Armenian geothermal springs

Համառոտագիր

Denaturing gradient gel electrophoresis (DGGE) of the partial 16S rRNA gene PCR amplicons was used to profile bacterial populations inhabiting in Arzakan, Jermuk and Karvachar geothermal springs. The spring in Arzakan was colonized by chemolithotrophic and phototrophic primary producers related to phyla Betaproteobacteria, Gammaproteobacteria and Epsilonproteobacteria including. The spring in Jermuk was colonized by phylotypes related to sulfur and hydrogen oxidizing chemolithotrophs belonging to Epsilonproteobacteria, along with a diversity of Bacteroidetes, Spirochaetes, Ignavibacteriae and Firmicutes. Representatives of the phylum Firmicutes were the major component of the bacterial community of Karvachar geothermal spring. Most of the sequences derived from DGGE bands were closely related to uncultivated microorganisms and according to BLASTn analysis shared less than 97% similarity with their closest matches in GenBank, indicating that studied springs harbor a unique community including novel microbial species.