Statistical analyses of enzyme kinetics: simple Michaelis-Menten and bi-bi ping-pong
Keywords:
Stationary kinetics – bi-bi ping-pong – multivariate linear regression – kinetic parameters – calculation
Abstract
Using multivariate linear regression approach, publicly available applications have been developed in Gauss 4.0 package for calculation of the kinetic parameters and their dispersions in the cases of simple Michaelis-Menten type and the bi-bi ping-pong type enzyme kinetics. The methods of derivation of corresponding equations of stationary kinetics, their linearization and derivation of analytic forms of kinetic parameters and their dispersions are presented.