The karyologic study of frogs (Pelophylax Ridibundus) hepatocytes living in different surrounding areas of Hrazdan river characterized by different degrees of pollution

Abstract

The main purpose of this research was studying the peculiar features of frogs (Pelophylax ridibundus) livers’ glandular cell nucleus and nucleolar (nucleolus) DNA quantity, mitotic activity, and the formation peculiarities of micronuclei in them, which live in Hrazdan rivers’ surrounding areas, characterized by different pollution degrees. Results of the study show that in the livers of frogs which live near Hrazdan River with medium or heavy pollution status have acquired adaptive, countervailing changes, which find their expression in proliferative activity of cells, in activation of transcription processes and increase in body mass. At the same time in areas described with the highest pollution – “bad” status/level a disruption of normal mitotic separation process of cells is observed, the result of which is the formation of micronuclei.