Peculiarities of acridine orange binding with DNA
Keywords:
DNA – acridine orange – AO-DNA complexes – absorption spectra – fluorescence spectra
Abstract
Absorption and fluorescence spectra of acridine orange (AO) complexes with DNA at 0.02 M ionic strength of the solution have been obtained. It was revealed that maximums of absorption spectra of AO complexes with DNA at low concentrations of DNA decrease and are shifted to longer wavelength region. At further enhancement of DNA concentration the absorption spectra of AO-DNA complexes start increasing. Moreover, in the spectra of AO-DNA complexes an isosbestic or pseudo-isosbestic point does not emerge. At the same conditions the fluorescence spectra of the complexes enhance as compared to AO fluorescence spectrum, which indicates the intercalation binding mode of AO with DNA.
