Analysis of fluorescence spectra of complexes of methylene blue with DNA
Keywords:
DNA – methylene blue – fluorescence spectrum – binding mode – intercalation – semi-intercalation – electrostatic binding
Abstract
The study of methylene blue (MB) binding to DNA has been carried out by fluorescence spectroscopy method. It was shown that on the fluorescence spectra of MB and its complexes with DNA two peaks exist at λ=583 nm and λ=682 nm wavelengths. Peak at λ=583 nm enhances with increasing of DNA concentration, but at λ=682 nm it decreases.The comparison of obtained data with the results received for DNA-EtBrcomplexes shows that MB binds to DNA by semiintercalative and electrostatic modes, despite the fact that this ligand is an intercalator
