Application of the vector рЕС-ХК99Е for the construction of recombinant strain-producers of L-arginine based on Brevibacterium flavum Biology

Abstract

Using Escherichia coli – Corynebacterium glutamicum shuttle expression vector pEC-XK99E, molecular cloning of the heterologous gene argJ of the thermophilic bacterium Geobacillus stearothermophilus, as well as of homologous genes argG and argH of Corynebacterium glutamicum was carried out in cells of coryneform bacterium Brevibacterium flavum. It was shown that expression of cloned arg genes from the strong promoter Ptrc (its activity is controlled by the protein repressor LacIq ) occurred regardless of the presence of transcription inducers lactose or IPTG in the medium.