Antibacterial and anti-phage activity of Plantago major L. raw material Biology

Abstract

Plantago major L. is a popular medicinal herb, which has been being used for treatment of various diseases in traditional health systems. The aim of present study was to examine the antibacterial and anti-phage activity of P. major ethanol extract. P. major fresh raw material extract was possessed of moderate antibacterial activity against Escherichia coli VKPM-M17, Salmonella typhimurium TA 100 and Staphilococcus aureus MDC 5233 bacterial strains. Anti-phage activity was evaluated by double overlay plaque assay against T4 phage of E. coli C-T4. Fresh, dried and frozen raw materials showed moderate antiviral activity: 1 mg/mL concentration of fresh row material ethanol extract caused 0.927Log10 reduction of phage units, while the frozen and dried plant ethanol extracts caused 0.875 Log10 and 0.821 Log10 reductions, respectively. Fresh raw material contains the highest concentration of vitamin C.