Cell viability and DNA damage in MRC5 and HeLa cell lines after histone H1 knockout by CRISPR-Cas9 genome editing technology Biology

Abstract

Chromatin research mainly focused on the core histones, whereas the role of H1.5 linker histone is poorly understood. Today CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeats associated protein 9) genome editing technology provides an opportunity to analyze functions of different genes introducing targeted loss-of function mutations. Here we demonstrate the role of histone H1.5 in cell viability and genome integrity in HeLa and MRC5 cells using trypan blue exclusion test and single-cell gel electrophoresis (comet) assay.